Cloning and Partial Sequencing of phac1 and phac2 Genes Encoding Poly (3-hydroxyalkanoate) Synthases from Pseudomonas aeroginusa PTCC 1310

Optimization of the Expression of Genes Encoding Poly (3-hydroxyalkanoate) Synthase from Pseudomonas aeruginosa PTCC 1310 in Escherichia coli

Over the years, the use of plastics has complicated the problem of disposal of solid wastes. One strategy to reduce plastic waste is the use of biodegradable plastics. A group of these plastics are polyhydroxyalkanoates (PHAs). To date more than 250 different microorganisms are known to synthesize and accumulate PHA. Most Pseudomonas strains are able to accumulate mcl-PHA. In previous studies, ...

optimization of the expression of genes encoding poly (3-hydroxyalkanoate) synthase from pseudomonas aeruginosa ptcc 1310 in escherichia coli

over the years, the use of plastics has complicated the problem of disposal of solid wastes. one strategy to reduce plastic waste is the use of biodegradable plastics. a group of these plastics are polyhydroxyalkanoates (phas). to date more than 250 different microorganisms are known to synthesize and accumulate pha. most pseu-domonas strains are able to accumulate mcl-pha. in previous studies,...

expression of genes encoding (3-hydroxyalkanoate) synthases from pseudomonas aeroginusa ptcc1310 in escherichia coli and optimization of condition for production of these enzymes

Poly‐3‐hydroxyalkanoate synthases from Pseudomonas putida U: substrate specificity and ultrastructural studies

The substrate specificity of the two polymerases (PhaC1 and PhaC2) involved in the biosynthesis of medium-chain-length poly-hydroxyalkanoates (mcl PHAs) in Pseudomonas putida U has been studied in vivo. For these kind of experiments, two recombinant strains derived from a genetically engineered mutant in which the whole pha locus had been deleted (P. putida U Δpha) were employed. These bacteria...

Cloning and molecular analysis of the Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) biosynthesis genes in Pseudomonas sp. strain 61-3.

Two types of polyhydroxyalkanoate (PHA) biosynthesis gene loci (phb and pha) of Pseudomonas sp. strain 61-3, which produces a blend of poly(3-hydroxybutyrate) [P(3HB)] homopolymer and a random copolymer (poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) [P(3HB-co-3HA]) consisting of 3HA units of 4 to 12 carbon atoms, were cloned and analyzed at the molecular level. In the phb locus, three open read...